avanti 830756p  (Croda International Plc)

Journal: Journal of Neurochemistry

Article Title: A Comprehensive Characterization of the Phospholipid and Cholesterol Composition of the Uncinate Fasciculus in the Human Brain: Evidence of Age‐Related Alterations

doi: 10.1111/jnc.70428

Figure Lengend Snippet: Mean totals and fraction‐specific characterization of FA classes. (A) Bar plot showing the mean concentration ± standard error for the totals of all measured phospholipid fractions, cholesterol, and the TL in ascending order ( n = 80). TL refers to the total FA, not just the FAs derived from phospholipids. Therefore, the FAs in the TL also come from glycolipids, diacylglycerols, and other lipid species. (B) Pie charts showing the mean relative percentage of MUFA (pink), omega‐6 (teal), omega‐3 (green), and SFA (purple) FAs for each phospholipid fraction as well as the TL ( n = 80). CerPCho, Sphingomyelin; ChoGpl, Choline glycerophospholipids; EtnGpl, Ethanolamine glycerophospholipids; MUFA, Monounsaturated fatty acid; PtdIns, Phosphatidylinositol; PtdSer, Phosphatidylserine; SFA, Saturated fatty acid; TL, Total lipid.

Article Snippet: An adapted Folch method was used to extract total lipids (TL) (Folch et al. ), at which point an internal standard cocktail containing di‐17:0 ChoGpl (Avanti Research; Catalog No: 850360), di‐17:0 EtnGpl (Avanti Research; Catalog No: 830756), and 5‐alpha cholestane (Sigma‐Aldrich; Catalog No: C8003) was added (Table S1).

Techniques: Concentration Assay, Derivative Assay

Journal: Journal of Neurochemistry

Article Title: A Comprehensive Characterization of the Phospholipid and Cholesterol Composition of the Uncinate Fasciculus in the Human Brain: Evidence of Age‐Related Alterations

doi: 10.1111/jnc.70428

Figure Lengend Snippet: UF phospholipids show no robust effect of group but pronounced effect of age. (A) Scatter plots with regression line showing significant relationships between age and concentration of FAs in ChoGpl (trend line in blue, n = 80), EtnGpl (trend line in purple, n = 80), PtdSer (trend line in green, n = 80), PtdIns (trend line in coral, n = 79), and TL (trend line in brown, n = 80). No significant age‐FA concentration relationships were identified for CerPCho ( n = 73). (B) Scatter plots with regression line showing significant relationships between age and relative percentage of FAs in ChoGpl (trend line in blue, n = 80), EtnGpl (trend line in purple, n = 80), PtdSer (trend line in green, n = 80), PtdIns (trend line in coral, n = 79), and TL (trend line in brown, n = 80). No significant age‐FA relative percentage relationships were identified for CerPCho ( n = 73). (C) Summary table showing age related changes in lipids separated by phospholipid fraction and TL. Arrows pointing upward indicate a positive coefficient for age, and arrows pointing downwards indicate a negative coefficient for age. FAs or summary metrics in bold indicate statistical significance after correction for multiple comparisons (BH‐corrected p < 0.05). CerPCho, Sphingomyelin; ChoGpl, Choline glycerophospholipids; EtnGpl, Ethanolamine glycerophospholipids; MUFA, Monounsaturated fatty acid; PtdIns, Phosphatidylinositol; PtdSer, Phosphatidylserine; PUFA, Polyunsaturated fatty acid; SFA, Saturated fatty acid; TL: Total lipid.

Article Snippet: An adapted Folch method was used to extract total lipids (TL) (Folch et al. ), at which point an internal standard cocktail containing di‐17:0 ChoGpl (Avanti Research; Catalog No: 850360), di‐17:0 EtnGpl (Avanti Research; Catalog No: 830756), and 5‐alpha cholestane (Sigma‐Aldrich; Catalog No: C8003) was added (Table S1).

Techniques: Concentration Assay

Journal: Journal of Neurochemistry

Article Title: A Comprehensive Characterization of the Phospholipid and Cholesterol Composition of the Uncinate Fasciculus in the Human Brain: Evidence of Age‐Related Alterations

doi: 10.1111/jnc.70428

Figure Lengend Snippet: Correlations between total lipids and myelin‐constituent proteins/genes. Correlation plots showing the relationships between total concentration (μg/g) of each lipid class (phospholipid fractions, the TL, and cholesterol) and (A) myelin‐constituent genes ( n = 43 overlapping subjects) and (B) proteins ( n = 42 overlapping subjects). Due to the exploratory nature of this analysis, p ‐values were not corrected for multiple comparisons. The color bar represents Pearson's correlation coefficient between −1 and 1, where negative coefficients are blue in color and positive coefficients are red in color. *** p < 0.001, ** p < 0.01, * p < 0.05. CerPCho, Sphingomyelin; ChoGpl, Choline glycerophospholipids; CNP, 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase; EtnGpl, Ethanolamine glycerophospholipids; MAG, Myelin‐associated glycoprotein; MBP, Myelin basic protein; MOBP, Myelin‐associated oligodendrocyte basic protein; MOG, Myelin oligodendrocyte glycoprotein; PLLP, Plasmolipin; PLP1, Proteolipid protein; PtdIns, Phosphatidylinositol; PtdSer, Phosphatidylserine; TL, Total lipid.

Article Snippet: An adapted Folch method was used to extract total lipids (TL) (Folch et al. ), at which point an internal standard cocktail containing di‐17:0 ChoGpl (Avanti Research; Catalog No: 850360), di‐17:0 EtnGpl (Avanti Research; Catalog No: 830756), and 5‐alpha cholestane (Sigma‐Aldrich; Catalog No: C8003) was added (Table S1).

Techniques: Concentration Assay